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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

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Goniofluorometer for characterization of fluorescent materials.

Silja Holopainen¹, Farshid Manoocheri, Erkki Ikonen

¹1Metrology Research Institute, Helsinki University of Technology, P. O. Box 3000, FI-02015 TKK, Finland. silja.holopainen@tkk.fi

|February 22, 2008

Summary

A novel goniofluorometer was developed for precise spectral measurements across diverse viewing and incident angles. This instrument measures bispectral luminescent radiance factors, offering unique capabilities in optical science.

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Area of Science:

Optical Physics
Materials Science
Spectroscopy

Background:

Accurate characterization of luminescent materials requires precise angular and spectral measurements.
Existing instrumentation may lack the flexibility for varied measurement geometries.

Purpose of the Study:

To design and construct a versatile goniofluorometer for advanced optical measurements.
To enable spectral analysis of luminescent radiance factors under controlled angular conditions.

Main Methods:

Development of a goniofluorometer with variable viewing angles (10-90 degrees) and incident angles (0-8 degrees).
Integration of capabilities for bispectral luminescent radiance factor measurements.
Operation within a broad wavelength range (250-800 nm).

Main Results:

Successful construction and validation of the goniofluorometer.
Demonstration of spectral measurements across a wide range of geometries.
Establishment of a unique measurement capability for bispectral luminescent radiance factors.

Conclusions:

The developed goniofluorometer provides unprecedented flexibility for spectral measurements in optics.
This device facilitates novel research in luminescent materials and optical phenomena.
The unique capabilities address a gap in current scientific instrumentation for angular-dependent spectral analysis.