Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic selection for seed yield enhances flax breeding efficiency.

Molecular breeding : new strategies in plant improvement·2026
Same author

QTL Associated with Partial Resistance to Root Rot of Field Pea Caused by <i>Aphanomyces euteiches</i> and <i>Fusarium avenaceum</i> in Two RIL Populations.

Plant disease·2026
Same author

Complete genome sequences of two effective nitrogen-fixing <i>Sinorhizobium medicae</i> strains isolated from <i>Medicago sativa</i> and <i>Melilotus albus</i> in Canada.

Microbiology resource announcements·2026
Same author

Elucidating putative novel functions of the AINTEGUMENTA-LIKE 7 transcription factor in clubroot resistance in Arabidopsis.

Plant molecular biology·2026
Same author

Complete genome sequences of <i>Rhizobium favelukesii</i> strains T136, T1470, and T1473 from Canada.

Microbiology resource announcements·2026
Same author

Avirulence genes identified through linkage mapping and region-specific association studies in the wheat leaf rust pathogen Puccinia triticina.

BMC genomics·2026

Related Experiment Video

Updated: Jul 7, 2026

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
11:50

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

Temporal gene expression profiling of the wheat leaf rust pathosystem using cDNA microarray reveals differences in

Bourlaye Fofana1, Travis W Banks, Brent McCallum

  • 1Cereal Research Centre, Winnipeg, MB, Canada.

International Journal of Plant Genomics
|February 22, 2008
PubMed
Summary
This summary is machine-generated.

Wheat leaf rust resistance involves differential gene expression. This study identified 192 genes, including those in photosynthesis and defense pathways, altered during compatible and incompatible interactions with Puccinia triticina.

More Related Videos

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae
06:58

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Published on: August 5, 2022

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens
06:12

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens

Published on: September 15, 2023

Related Experiment Videos

Last Updated: Jul 7, 2026

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
11:50

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae
06:58

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Published on: August 5, 2022

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens
06:12

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens

Published on: September 15, 2023

Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Wheat Genetics

Background:

  • Leaf rust, caused by Puccinia triticina, is a significant disease in wheat.
  • Understanding wheat's genetic response to pathogens is crucial for developing resistant cultivars.

Purpose of the Study:

  • To identify wheat genes differentially expressed during leaf rust infection.
  • To elucidate host defense mechanisms against Puccinia triticina.

Main Methods:

  • Construction of a custom cDNA microarray with 7728 wheat expressed sequence tags (ESTs).
  • Inoculation of wheat cultivar RL6003 with compatible (TJB) and incompatible (BBB) Puccinia triticina strains.
  • Gene expression analysis at 3, 6, 12, and 24 hours post-inoculation using the custom microarray.

Main Results:

  • 192 genes exhibited significantly altered expression between compatible and incompatible interactions.
  • Affected pathways include photosynthesis, reactive oxygen species production, ubiquitination, signal transduction, and the shikimate/phenylpropanoid pathway.
  • Differential expression patterns suggest coordinated defense responses in wheat.

Conclusions:

  • Wheat cultivar RL6003 employs diverse metabolic pathways for defense against incompatible leaf rust pathogens.
  • Gene expression profiling provides insights into wheat-pathogen interactions.
  • The identified genes offer potential targets for breeding rust-resistant wheat varieties.