Related Experiment Video

Updated: Jul 7, 2026

Colon Ascendens Stent Peritonitis (CASP) - a Standardized Model for Polymicrobial Abdominal Sepsis
06:45

Colon Ascendens Stent Peritonitis (CASP) - a Standardized Model for Polymicrobial Abdominal Sepsis

Published on: December 18, 2010

Lipopolysaccharide binding protein and sCD14 are not produced as acute phase proteins in cardiac surgery

Manuela Kudlova1, Pavel Kunes, Martina Kolackova

  • 1Institute of Clinical Immunology and Allergology, Charles University in Prague, School of Medicine and University Hospital in Hradec Králové, 500 05 Hradec Kralove , Czech Republic.

Mediators of Inflammation
|February 22, 2008
PubMed

Insights

Serum levels of lipopolysaccharide binding protein (LBP) and soluble CD14 (sCD14) increase after cardiac surgery, with similar elevations observed in both on-pump and off-pump procedures. These markers do not appear to be acute phase proteins in this context.

Area of Science:

  • Biochemistry
  • Immunology
  • Cardiovascular Surgery

Background:

  • Cardiac surgery can trigger inflammatory responses.
  • Lipopolysaccharide binding protein (LBP) and soluble CD14 (sCD14) are key components of the innate immune system.
  • Understanding their behavior during cardiac surgery is crucial for assessing patient outcomes.

Purpose of the Study:

  • To investigate the dynamic changes in serum LBP and sCD14 levels in patients undergoing coronary artery bypass grafting (CABG).
  • To compare these changes between on-pump and off-pump surgical techniques.

Main Methods:

  • A randomized study involving 34 patients undergoing CABG, divided into on-pump and off-pump groups (17 each).
  • Serum LBP and sCD14 levels were quantified using enzyme-linked immunosorbent assay (ELISA).
  • Measurements were taken at various postoperative time points.

Main Results:

  • Serum LBP levels progressively increased postoperatively, peaking on day 3, with no significant difference between on-pump and off-pump groups.
  • Serum sCD14 levels also significantly increased from day 1 to day 7 post-surgery in both groups, without significant inter-group differences.
  • No correlations were found between LBP, sCD14, and inflammatory markers like IL-6, CRP, or PTX3.

Conclusions:

  • Serum LBP and sCD14 levels are elevated following cardiac surgery, irrespective of the surgical technique (on-pump vs. off-pump).
  • The observed elevation suggests these molecules are involved in the systemic response to cardiac surgery.
  • LBP and sCD14 do not appear to function as acute phase proteins in this specific clinical setting.
Abstract

Related Concept Videos

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers01:19

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...
Blood Studies for Cardiovascular System I: Cardiac Biomarkers01:20

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...