Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 7, 2026

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle
05:05

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle

Published on: November 4, 2011

Poly(L-lysine)-modified iron oxide nanoparticles for stem cell labeling.

Michal Babic1, Daniel Horák, Miroslava Trchová

  • 1Institute of Macromolecular Chemistry, v v i, Academy of Sciences of the Czech Republic, Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic.

Bioconjugate Chemistry
|February 22, 2008
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of Oleic Acid-Modified Hydroxyapatite Polymer Nanocomposites for Regenerative Medicine Applications.

Applied spectroscopy·2026
Same author

Imaging-guided photodynamic control of autophagy-apoptosis switching in breast cancer using hypericin-functionalized upconverting nanoparticles.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Surface characterization of curcumin-visualized latent fingerprints on metal surfaces.

Analytical and bioanalytical chemistry·2026
Same author

Upconversion Colloid for Tracking Cellular Uptake of Nanoparticles.

International journal of molecular sciences·2026
Same author

Single-Nanoparticle Luminescence Nanothermometers with Enhanced Sensitivity in Physiological Temperature Range.

ACS omega·2026
Same author

Bisphosphonate-Modified Polymer-Coated NaYF<sub>4</sub>:Yb,Er,Pr Upconverting Nanoparticles for Cell Imaging: Synthesis, Physicochemical Characterization and Biosafety.

Journal of biomedical materials research. Part B, Applied biomaterials·2025

New poly( L-lysine) (PLL) coated iron oxide nanoparticles show over 92% cellular uptake in mesenchymal stem cells (MSCs). These nanoparticles enable effective in vitro and in vivo cell imaging via magnetic resonance (MR) imaging.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Cell Biology

Background:

  • Iron oxide nanoparticles (IONPs) are crucial for biomedical applications, including cell tracking and imaging.
  • Enhancing intracellular uptake of IONPs is essential for improving their efficacy in diagnostic and therapeutic strategies.
  • Surface modification of IONPs can significantly influence their interaction with cells and biological environments.

Purpose of the Study:

  • To develop novel surface-modified iron oxide nanoparticles with enhanced intracellular uptake.
  • To investigate the effect of poly( L-lysine) (PLL) coating on IONP cellular internalization.
  • To evaluate the potential of PLL-modified IONPs for in vitro and in vivo cell imaging using magnetic resonance (MR) imaging.

Main Methods:

  • Synthesis of IONPs via co-precipitation and surface oxidation.

More Related Videos

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
09:06

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Published on: March 31, 2008

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
08:26

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

Related Experiment Videos

Last Updated: Jul 7, 2026

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle
05:05

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle

Published on: November 4, 2011

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
09:06

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Published on: March 31, 2008

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
08:26

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

  • Modification of IONPs with poly( L-lysine) (PLL) of varying molecular weights.
  • Characterization of nanoparticles using TEM, DLS, FTIR, and UV-vis spectroscopy.
  • Assessment of cellular uptake in rat bone marrow stromal cells (rMSCs) and human mesenchymal stem cells (hMSCs).
  • In vitro and in vivo MR imaging of PLL-IONP labeled cells.

    • Main Results:

    • Synthesized IONPs were approximately 6 nm in diameter and successfully coated with PLL.
    • PLL modification significantly enhanced cellular uptake, achieving over 92% efficiency with an optimal PLL molecular weight (388,000).
    • PLL-IONP labeled MSCs were successfully imaged in vitro and in vivo, demonstrating their potential as MR contrast agents.
    • PLL-IONPs showed higher intracellular uptake efficiency compared to the commercial control (Endorem).

    Conclusions:

    • Surface modification with poly( L-lysine) is an effective strategy to enhance the intracellular uptake of iron oxide nanoparticles.
    • PLL-modified IONPs exhibit excellent potential for labeling mesenchymal stem cells for advanced cell tracking and MR imaging applications.
    • The developed nanoparticles offer a promising tool for in vitro and in vivo biomedical imaging and cell therapy monitoring.