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Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

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Micellar electrokinetic chromatography on microchips.

Fumihiko Kitagawa¹, Koji Otsuka

¹Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, Japan. fkitagawa@mbox.kudpc.kyoto-u.ac.jp

Journal of Separation Science

|February 23, 2008

Summary

This summary is machine-generated.

This review covers advancements in microchip micellar electrokinetic chromatography (MCMEKC) since 1995. It details high-throughput analytical systems, on-chip sample manipulation, and applications for biogenic compounds.

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Area of Science:

Analytical Chemistry
Separation Science
Microfluidics

Background:

Micellar electrokinetic chromatography (MEKC) offers high separation efficiency.
Microchip electrophoresis (MCE) provides high-speed separations and integration capabilities.
Chip-based systems enable multidimensional separations and on-chip sample manipulation.

Purpose of the Study:

To review methodological and instrumental developments in microchip micellar EKC (MCMEKC) from 1995.
To highlight the integration of sample manipulation techniques on MEKC microchips.
To present applications of MCMEKC for analyzing biogenic compounds.

Main Methods:

Review of literature on microchip micellar EKC (MCMEKC) developments.
Description of various 2-D separation microchip configurations.
Analysis of integrated on-chip sample manipulation techniques like preconcentration and reactions.

Main Results:

MCMEKC combines high efficiency (MEKC) with high-speed separation (MCE) for high-throughput systems.
On-chip sample manipulation, including preconcentration, enhances sensitivity and allows mechanism elucidation.
Applications span biogenic compounds: amino acids, peptides, proteins, biogenic amines, DNA, and oestrogens.

Conclusions:

MCMEKC offers a powerful platform for high-performance, high-throughput analysis.
Integration of sample manipulation on microchips significantly improves analytical capabilities.
MCMEKC is a versatile technique for the analysis of diverse biogenic compounds.