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A novel nanobiocomposite based glucose biosensor using neutral red functionalized carbon nanotubes.
D R Shobha Jeykumari1, S Sriman Narayanan
1Department of Analytical Chemistry, School of Chemical Sciences, University of Madras, Guindy Campus, Chennai 600 025, India.
Biosensors & Bioelectronics
|February 26, 2008
Summary
A novel glucose biosensor combines glucose oxidase (GOx) with carbon nanotubes (CNTs) and neutral red (NR) for sensitive glucose detection. This nanobiocomposite offers a low potential, rapid response, and excellent stability for electrochemical applications.
Area of Science:
- Electrochemistry
- Biosensors
- Nanomaterials
Background:
- Development of sensitive and selective glucose biosensors is crucial for diagnosing and managing diabetes.
- Existing biosensors often face challenges with stability, response time, and detection limits.
- Carbon nanotubes (CNTs) offer excellent electrical conductivity and high surface area for biosensor development.
Purpose of the Study:
- To develop and characterize a novel glucose biosensor utilizing multiwalled carbon nanotubes (MWNTs) functionalized with neutral red (NR) and glucose oxidase (GOx).
- To evaluate the electrochemical performance and analytical characteristics of the MWNT-NR-GOx-Nf nanobiocomposite modified electrode for glucose determination.
Main Methods:
- Covalent immobilization of neutral red (NR) onto carboxylic acid groups of MWNTs via carbodiimide reaction.
- Preparation of the MWNT-NR-GOx-Nf nanobiocomposite by mixing GOx and Nafion (Nf) with functionalized MWNTs.
- Electrochemical characterization using cyclic voltammetry and electrochemical impedance spectroscopy.
Main Results:
- The nanobiocomposite exhibited excellent electrocatalytic activity for the reduction of hydrogen peroxide.
- The developed glucose biosensor demonstrated a wide determination range (1 x 10(-8) to 1 x 10(-3)M) with a low detection limit (3 x 10(-9)M).
- The biosensor showed a rapid response time (4s), good stability, and anti-interference properties.
Conclusions:
- The MWNT-NR-GOx-Nf nanobiocomposite biosensor offers a highly efficient platform for selective glucose detection.
- The combination of CNTs, NR, and GOx significantly enhances electrocatalytic activity and sensor performance.
- This nanobiocomposite system holds potential for immobilizing various enzymes in bioelectrochemical applications.
