Excitable dynein model with multiple active sites for large-amplitude oscillations and bend propagation in flagella

The formal excitable dynein model proposed by Murase et al. (1989, J. theor. Biol. 139, 413-430) is modified to produce large-amplitude oscillations and excitability. The present model assumes that (i) each dynein arm has multiple active sites, which are distributed along most of the 24-nm distance between adjacent B-subtubule attachment sites; and (ii) any given dynein molecule tends to produce force continuously during interdoublet sliding in one direction and to produce little force during sliding in the opposite direction. Since no sliding motion occurs without superthreshold perturbations in the form of the sliding displacement, this new model also possesses an excitable nature. Once passive elastic components (e.g. nexin links and radial spokes) are incorporated into this model, oscillations with large amplitudes result. To test the ability of the model for bend propagation without a curvature-control mechanism, forced oscillations are applied to the basal end of the flagellum by the sliding displacement. It is found that bend propagation can occur even in the absence of a curvature-control mechanism.