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Updated: Jul 7, 2026
07:40
Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
Published on: July 31, 2015
Improvement of transformation efficiency by bioactive-beads-mediated gene transfer using DNA-lipofectin complex as
Tomoko Murakawa1, Shin'ichiro Kajiyama, Tomohiko Ikeuchi
1Sasebo National College of Technology, Sasebo, Nagasaki, Japan. murakawa@post.cc.sasebo.ac.jp
Journal of Bioscience and Bioengineering
|February 26, 2008
Abstract:
The efficiency of bioactive-beads-mediated plant transformation was improved using DNA-lipofectin complex as the entrapped genetic material instead of naked DNA used in the conventional method. In the improved method, beads aggregated and formed clusters around the protoplasts resulting in a 4-fold higher transformation efficiency than that by the conventional method.
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