Platelet-activating factor-acether is a component of human follicular fluid
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M L Amiel1, J Testart, J Benveniste
1Institut National de la Santé et de la Recherche Médicale (INSERM), Clamart, France.
Objective:
Platelet-activating factor-acether (PAF-acether) presence was investigated in 27 human follicular fluids (FFs).
Design:
Aggregation of washed rabbit platelets was used to measure PAF-acether. Data were compared using Student's t-test.
Setting:
Follicular fluids came from the in vitro fertilization program at Antoine Béclère Hospital, and PAF-acether was assayed at Institut National de la Santé et de la Recherche Médicale, Unités 187 and 200, Clamart, France.
Patients, Participants:
The study concerned five infertile women 29 to 39 years of age.
Interventions:
Ovaries were stimulated with human menopausal gonadotropin under gonadotropin-releasing hormone agonist (GnRH-a) action.
Main Outcome Measures:
The height of platelet aggregation was compared between FFs and synthetic PAF-acether.
Results:
Mean FF concentration of PAF-acether was 1,367 to 3,467 pg/mL among women. Values were higher for patients in a long than in a short GnRH-a protocol (P less than 0.05). However, PAF-acether concentration was not related to fertilization rate.
Conclusions:
Platelet-activating factor-acether is possibly involved in oocyte release from the follicle, a process occulted by follicular puncture.