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ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
Published on: June 9, 2011
Detection of adamantane-resistant influenza on a microarray.
Michael B Townsend1, James A Smagala, Erica D Dawson
1Department of Chemistry and Biochemistry, The University of Colorado at Boulder, UCB #215, Boulder, CO 80309, USA.
Summary
A new antiviral resistance-chip (AVR-Chip) rapidly detects adamantane resistance in influenza A viruses. This cost-effective microarray accurately identifies key M2 protein mutations, aiding treatment decisions.
Area of Science:
- Virology
- Genomics
- Biotechnology
Background:
- Influenza A virus rapidly develops resistance to antiviral drugs, complicating treatment.
- Emerging resistance necessitates novel diagnostic tools for timely intervention.
Purpose of the Study:
- To develop a cost-effective, low-density microarray for detecting adamantane resistance in influenza A.
- To identify specific mutations in the M2 protein linked to antiviral resistance.
Main Methods:
- Utilized functional genomics and microarray technology to design a DNA microarray.
- The microarray targets two common M2 protein mutations: V27A and S31N.
Main Results:
- The antiviral resistance-chip (AVR-Chip) achieved 95% success in detecting target mutations in 22 influenza isolates.
- Artificial neural network analysis of larger datasets yielded a 94% correct identification rate for V27A and S31N mutations.
Conclusions:
- The AVR-Chip offers a rapid screening method for influenza virus adamantane sensitivity.
- The microarray approach is adaptable for detecting resistance to other antiviral chemotherapeutics.
