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[Separation, purification and spectrum analysis of total flavonoids from Cercis chinensis]
Mei-yi Xu1, Ya-li Han, Guang-zhi Dong Ye
1Analysis Center, Guangdong Medical College, Zhangjiang 524023, China. myxugdmc@126.com
Objective:
To separate, purify total flavonoids from Cercis chinensis Bge and analyze their flavonoid aglycones and content by spectrum method.
Methods:
The total flavonoids from Cercis chinensis Bge were extracted by 15 volume of 55% ethyl alcohol and hot water refluence at 100 degrees C and separated, purified by polyamide resin chromatography. Based on reference substances of rutin, quercetin, kaempferol, it was analyzed and detected by UV-Vis spectroscope at 200-600 nm and by KBr infrared spectroscope at 400-4000 cm(-1).
Results:
Average extraction rate of crude total flavonoids was 7.32% , and extraction rate of purified total flavonoids was 2.20%. The results of UV-Vis and IR scanning spectrum showed that purified total flavonoids from Cercis chinensis Bge contained of 3 kinds of flavonoid aglycones of rutin, quercetin, kaempferol, and their content was 0.8271, 0.2169, 0.3007 mg/ml respectively.
Conclusion:
Content of total flavonoid aglycones in purified flavonoids of Cercis chinensis Bge was 24.30%, and content of total flavonoid was 0.5346%.