[Study of differentation of HL-60 cells by induction of SPGL]
Hua-zhong Yang1, Jian-hui Zhang, Kui Song
1Medical College of Hunan Normal University, Hunan Changsha 410006, China.
Objective:
To study the effect of differentiation of HL-60 cells by induction of SPGL.
Methods:
Cell differentiation was analyzed by using Wrigh-Giemsa staining to observe the morphology changes of cells with microscope, NBT reductant test, surface differentiation antigen (CD11b and CD14) test in HL-60 cells treated by different doses (1 x 10(-5) - 5 x 10(-4) ng/ml) of SPGL at the 3th day.
Results:
The morphological changes showed cell differentiation characteristics; NBT reductant was significantly increased, and the number of NBT positive cells were related to dose of SPGL (in dose-dependent manner); Expression of CD11b and CD14 increased obviously.
Conclusion:
The results indicate that the effect of differentiation of HL-60 cells by induction of SPGL and it induces the cells to differentiate along the monocyte and granulocyte lineage.
