Related Experiment Video

Updated: Jul 7, 2026

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

Refractive-index effect on spectral transmission factor of liquids on double-beam molecular absorption spectrometry

O D Soares

    Applied Optics
    |February 28, 2008
    PubMed

    More Related Videos

    Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing08:12

    Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

    Published on: March 13, 2013

    Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks10:31

    Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks

    Published on: May 8, 2015

    Related Concept Videos

    UV–Vis Spectroscopy: Beer–Lambert Law01:09

    UV–Vis Spectroscopy: Beer–Lambert Law

    The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
    Spectrophotometry: Introduction01:16

    Spectrophotometry: Introduction

    Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
    The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
    IR Spectrum01:19

    IR Spectrum

    When infrared (IR) radiation passes through a molecule, the bonds stretch or bend by absorbing the radiation. This absorption creates the molecule's absorption spectrum, which is the plot of its percentage transmittance versus wavenumber.
    Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0% (complete...
    Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

    Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

    Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
    The ATR process begins by directing a beam...
    UV–Vis Spectrometers01:14

    UV–Vis Spectrometers

    The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
    Infrared (IR) Spectroscopy: Overview01:09

    Infrared (IR) Spectroscopy: Overview

    When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
    Different compounds display unique properties due to their...