Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardiorenal Syndrome and Depressive Symptoms: Exploring the Mood-Kidney Link With Heart Failure Risk in a Post-hoc Analysis of SPRINT.

Kidney medicine·2026
Same author

TESTING FOR THE CAUSAL MEDIATION EFFECTS OF MULTIPLE MEDIATORS USING THE KERNEL MACHINE DIFFERENCE METHOD IN GENOME-WIDE EPIGENETIC STUDIES.

The annals of applied statistics·2026
Same author

SNAP MRI reveals association between distal cerebral arterial flow and cognitive function in an aging population.

Magnetic resonance imaging·2026
Same author

Metabolic engineering reveals LUP5 as a determinant of saponin composition and insect feeding preference in Barbarea vulgaris.

Plant physiology·2026
Same author

N6-methyladenosine (m6A)-circHECA facilitates the differentiation of SHF stem cells into hair follicle lineage through miR-449a-5p/LEF1 mediated Wnt/β-catenin pathway in cashmere goats.

Animal bioscience·2026
Same author

Associations between Perceived Costs and Rewards of Sun Protection and Sun Safety Practices among Rural and Urban High School Students.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026

Related Experiment Video

Updated: Jul 7, 2026

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
04:25

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach

Published on: August 8, 2025

DBMLoc: a Database of proteins with multiple subcellular localizations.

Song Zhang1, Xuefeng Xia, Jincheng Shen

  • 1MOE Key Laboratory of Bioinformatics, State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China. zhangsong04@mails.tsinghua.edu.cn

BMC Bioinformatics
|February 29, 2008
PubMed
Summary
This summary is machine-generated.

Researchers created DBMLoc, a database for proteins with multiple subcellular localizations. This resource aids in understanding protein function by cataloging proteins found in various cellular compartments.

More Related Videos

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
09:02

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Published on: April 20, 2018

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
11:04

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Published on: December 24, 2016

Related Experiment Videos

Last Updated: Jul 7, 2026

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
04:25

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach

Published on: August 8, 2025

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
09:02

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Published on: April 20, 2018

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
11:04

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Published on: December 24, 2016

Area of Science:

  • Proteomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Subcellular localization is crucial for understanding protein function.
  • Proteins with multiple localizations offer significant insights into complex cellular processes.
  • Over 35% of proteins may exhibit dual or multiple subcellular localizations.

Purpose of the Study:

  • To develop a comprehensive database for proteins with multiple subcellular localizations.
  • To provide a centralized resource for researchers studying protein function and localization.

Main Methods:

  • Developed DBMLoc, a database containing 10,470 entries with multiple subcellular localization annotations.
  • Collected annotations from primary protein databases, specialized localization databases, and scientific literature.
  • Cross-referenced entries with Gene Ontology (GO) annotations and SwissProt, including protein-protein interactions.
  • Classified proteins into 12 major subcellular localization categories using GO hierarchy and original annotations.

Main Results:

  • The initial release of DBMLoc includes 10,470 proteins annotated with multiple subcellular localizations.
  • The database integrates data from diverse sources, ensuring comprehensive annotation.
  • Provides tools for searching, downloading data, and performing sequence BLAST analysis.

Conclusions:

  • DBMLoc serves as a valuable, freely accessible resource for studying proteins with multiple subcellular localizations.
  • The database facilitates research into the functional implications of protein dual localization.
  • DBMLoc is available at http://www.bioinfo.tsinghua.edu.cn/DBMLoc/index.htm.