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Updated: Jan 20, 2026
Author Spotlight: Studying Cardiac Cell-Matrix Interactions In Vitro
Published on: March 22, 2024
[Increased expression of extracellular matrix metalloproteinase inducer through interaction between fibroblasts and
You-wei Zhang1, Hong Deng, Ping-sheng Chen
1Department of Pathology, School of Basic Medical Sciences, Southeast University, Nanjing 210009, China.
Objective:
To investigate the expression of extracellular matrix metalloproteinase inducer gene (EMMPRIN) regulated by the interactions between fibroblasts and colon cancer cells, and to study its role in the invasion and metastasis of colon cancer.
Methods:
Colon cancer cells (SW480) were co-cultured with fibroblasts (HELF) in RPMI 1640 media for 0, 12, 24 and 48 hours. The expression of EMMPRIN in SW480 cells and HELF cells was documented by RT-PCR and immunocytochemistry.
Results:
The mRNA and protein expressions of EMMPRIN in SW480 cells were remarkably increased by the co-culturing with HELF cells. Although without the endogenous expression, HELF cells began to express EMMPRIN in a time-dependent manner after being co-cultured with SW480 cells.
Conclusions:
Intercellular interactions between colon cancer cells and fibroblasts not only up-regulate the EMMPRIN expression in SW480 cells, but also induce its expression in HELF cells. Such interactions may play a crucial role in the invasion and metastasis of colon cancer.
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