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[Determination of beta-eudesmol in rhizome of Atractylodes lancea by RP-HPLC]
Yan-Ming Chen1, Gui-Xin Chou, Zheng-Tao Wang
1Key Laboratory of Standardization of Chinese Medicine, Ministry of Education, Institutes of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Objective:
To develop a RP-HPLC method for determination of beta-eudesmol in rhizome of Atractylodes lancea, and to provide valuble data for quality control of A. lancea.
Method:
The samples were separated on an Inertsil ODS-3 (4.6 mm x 250 mm, 5 microm) column with the mobile phase of acetonitrile-water (68:32). Flow rate was 1.0 mL x min(-1). The detection wavelength was set at 200 nm. Column temperature was 25 degrees C.
Result:
The contents of beta-eudesmol determinated was 0.833-4.466 mg x g(-1), The linear range of beta-eudesmol was 0.048-1.200 microg (r = 0.999 9), the average recovery was 99.3%, RSD was 1.4% (n = 9).
Conclusion:
The method for quantitation of beta-eudesmol in A. lancea was accurate and reliable, which can be used to evaluate the quality of rhizome of A. lancea.
