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Updated: Jul 7, 2026

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
Published on: June 29, 2011
Functional cloning of genes regulating apoptosis in neuronal cells
Roberta Visconti1, Luciano D'Adamio
1Ceinge Biotecnologie Avanzate S.C.ar.l., Napoli, Italy.
Abstract:
Here, we describe the use of a functional cloning approach, based on the screen of a genome-wide short hairpin RNA (shRNA) library, to identify novel genes regulating apoptosis in neuronal cells. Apoptosis is induced by doxorubicin and is detected with a fluorometric caspase 3 assay. Moreover, we describe also the screen of the library to identify genes regulating the processing f the beta-amyloid (Abeta) precursor protein (APP), the protein associated with the pathogenesis of lzheimer's disease. The levels of the peptide Abeta, produced by the APP processing, are detected with ELISA based on the innovative Delfia method developed by PerkinElmer.
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