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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

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Updated: Jul 7, 2026

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

A high-throughput hybridoma selection method using fluorometric microvolume assay technology.

Rozanne Lee¹, Mylinh Tran, Mark Nocerini

¹Medarex, Inc., Milpitas, California, USA.

Journal of Biomolecular Screening

|March 4, 2008

Summary

This study introduces a new cell-based assay using fluorometric microvolume assay technology (FMAT) for identifying specific monoclonal antibodies (mAbs). The FMAT method efficiently detects antibodies binding to cell surface antigens, crucial for therapeutic development.

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Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Area of Science:

Biotechnology
Immunology
Drug Discovery

Background:

Monoclonal antibodies (mAbs) are vital reagents and therapeutics due to their antigen specificity.
Traditional methods like ELISA struggle to identify antibodies recognizing native cell surface antigens.
Selecting antigen-specific antibodies is critical for mAb development.

Purpose of the Study:

To develop a high-throughput, cell-based assay for identifying antibodies against cell surface antigens.
To overcome limitations of existing methods in detecting antibodies for native cell surface targets.

Main Methods:

Development of a homogeneous assay using fluorometric microvolume assay technology (FMAT).
Utilized antigen-expressing and antigen-negative cells to differentiate specific from non-specific binding.
Assessed antibody binding to cell surface antigens in a high-throughput format.

Main Results:

The FMAT assay demonstrated high sensitivity, detecting antibodies as low as 5 ng/mL.
The method could detect low cell surface antigen expression (9000 copies/cell).
FMAT provides a sensitive and homogeneous approach for antibody detection.

Conclusions:

The FMAT assay is a sensitive and homogeneous method for detecting antibody binding to cell surface antigens.
This technology is suitable for high-throughput screening of hybridomas in monoclonal antibody development.