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Updated: Jul 7, 2026

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation
Published on: September 29, 2019
High-affinity sulfonated materials with transition metal counterions for enhanced protein separation in dual-layer
Yi Li1, Tai-Shung Chung, Sui Yung Chan
1Department of Chemical and Biomolecular Engineering, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore.
Abstract:
High-affinity membrane materials have been successfully synthesized through a combination of the polymer sulfonation reaction with transition metal counterion exchange treatment. This type of promising materials were embodied for the first time with the aid of dual-layer hollow fiber technology for protein separation. Three types of immobilized metal affinity membranes (Cu(2+), Ni(2+) and Zn(2+) forms) were developed in this work and they all exhibited enhanced protein separation performance compared to the as-spun hollow fiber in H(+) form due to the strong affinity between transition metal counterions and target protein molecules. Ultimately, the high-purity target protein (>99%, w/w) could be achieved via the membrane in Cu(2+) form.
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