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Updated: Jul 7, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
A novel and rapid method for synthesizing positive controls and standards for quantitative PCR
Maude M David1, Amy R Sapkota, Pascal Simonet
1Environmental Microbial Genomics Group, Microsystems and Microbiology, Laboratoire Ampère, UMR CNRS 5005, Ecole Centrale de Lyon, Université de Lyon, 36 avenue Guy de Collongue 69134 Ecully cedex, France.
Abstract:
We developed and tested a method to produce DNA standards and controls for quantitative PCR by designing and performing partial hybridization of long oligonucleotides before double stranded DNA fragments were synthesized and subsequently amplified by conventional PCR. This approach does not require any natural DNA template. Applications include the production of standards, which cannot be easily produced from DNA extracted from bacteria or plants.
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