Related Experiment Video
Updated: Jul 7, 2026
Spatula Montevideo Device for the Vitrification of Mammalian Embryos
Published on: June 6, 2025
Blastocyst rate and live births from vitrification and slow-cooled two-cell mouse embryos
Jennifer E Graves-Herring1, William R Boone
1Department of Obstetrics and Gynecology, Division of Reproduction Endocrinology and Infertility, Greenville Hospital System University Medical Group, Greenville, South Carolina 29605, USA.
Objective:
The purpose of this study was to develop a closed vitrification system, compare vitrification to a slow-cooled cryopreservation method, and compare the pup rate between both methods using two-cell mouse embryos.
Design:
Randomized, prospective animal study.
Setting:
Hospital-based IVF practice.
Animal(S):
B6C3F1 mouse embryos.
Intervention(S):
Two-cell mouse embryos were cryopreserved using a slow-cooled or vitrification method and then thawed at a later date. The embryos were cultured and transferred to recipient females.
Main Outcome Measure(S):
Embryos were observed for blastocyst rate and pups were observed for phenotypic anomalies and weighed at 30, 60, and 90 days after birth.
Result(S):
Neither the blastocyst rate, pup rate, nor pup weights were significantly different when the two cryopreservation methods were compared.
Conclusion(S):
Because there were no differences in blastocyst rates, pup rates, or pup weights, we plan to further investigate the potential effects of vitrification on genotypic damage via the Comet Assay.
