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Updated: Jul 7, 2026
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Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
Published on: July 20, 2019
Conventional and immunoelectron microscopy of mitochondria
Edward M Perkins1, J Michael McCaffery
1Integrated Imaging Center, Department of Biology, The Johns Hopkins University, Baltimore, MD, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2008
Summary
Electron microscopy (EM) and immunoelectron microscopy (IEM) reveal mitochondrial protein localization and dynamics. This chapter details EM and IEM protocols for analyzing mitochondria in yeast and mammalian cells.
Area of Science:
- Cell Biology
- Microscopy Techniques
Background:
- Electron microscopy (EM) is crucial for understanding eukaryotic cell organization.
- EM has provided key insights into mitochondria structure and function since 1944.
- Immunoelectron microscopy (IEM) is vital for studying mitochondrial dynamics and protein localization.
Purpose of the Study:
- To detail conventional EM and IEM methods for mitochondrial analysis.
- To present protocols successfully utilized in the authors' research center.
- To focus on mitochondria in yeast and mammalian cells.
Main Methods:
- Conventional Electron Microscopy (EM)
- Immunoelectron Microscopy (IEM)
- Detailed protocol presentation for yeast and mammalian cells.
Main Results:
- Demonstration of key mitochondrial protein localizations (e.g., Dnm1p, Fiz1p, Mgm1p) in yeast.
- Insights into mitochondrial dynamics through IEM.
- Successful application of EM and IEM for detailed mitochondrial examination.
Conclusions:
- EM and IEM are powerful tools for dissecting mitochondrial structure, function, and dynamics.
- The provided protocols enable detailed analysis of mitochondria in various cell types.
- This work contributes practical methods for advancing mitochondrial research.
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