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Related Experiment Video

Updated: Jul 7, 2026

High Throughput Single-cell and Multiple-cell Micro-encapsulation
16:19

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

Microfluidic high-throughput encapsulation and hydrodynamic self-sorting of single cells.

Max Chabert1, Jean-Louis Viovy

  • 1Institut Curie, Section de Recherche, 26, rue d'Ulm, 75005 Paris, France.

Proceedings of the National Academy of Sciences of the United States of America
|March 5, 2008
PubMed
Summary

This study introduces a hydrodynamic method for high-throughput single-cell encapsulation and sorting into picoliter droplets. The cost-effective technique achieves over 70% efficiency without complex equipment, enabling rapid cell enrichment.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • High-throughput single-cell analysis requires efficient methods for isolating individual cells.
  • Current methods often involve complex, costly, or slow processes.
  • Microfluidic devices offer potential for precise cell handling.

Purpose of the Study:

  • To develop a purely hydrodynamic method for high-throughput single-cell encapsulation and droplet self-sorting.
  • To achieve efficient and cost-effective cell isolation and enrichment using microfluidics.
  • To demonstrate the application of this method for isolating cancer cells from whole blood.

Main Methods:

  • Utilized a flow-focusing geometry with cell-triggered Rayleigh-Plateau instability for encapsulation.
  • Employed hydrodynamic mechanisms including lateral drift and sterically driven dispersion for droplet self-sorting.

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Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

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A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
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A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

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Last Updated: Jul 7, 2026

High Throughput Single-cell and Multiple-cell Micro-encapsulation
16:19

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

  • Integrated encapsulation and sorting on-chip in continuous flow without active components.

    • Main Results:

    • Achieved high-throughput encapsulation and sorting at rates up to 160 cells per second.
    • Demonstrated successful encapsulation and sorting of 70-80% of cells into single-cell droplets with <1% empty droplet contamination.
    • Showcased >10,000-fold enrichment of cancerous lymphocytes from whole blood samples.

    Conclusions:

    • The developed hydrodynamic method provides a robust, cost-effective, and efficient approach for single-cell encapsulation and sorting.
    • This technique is suitable for integration into microfluidic systems for various cell biology and molecular biology applications.
    • The method shows significant promise for applications like cancer cell isolation and enrichment from complex biological mixtures.