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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: Jun 23, 2026

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
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On-Chip Magnetic Extraction of Circulating Cell-Free DNA from Biological Samples.

Karla Perez-Toralla1, Iago Pereiro2,3,4, Sonia Garrigou5

  • 1Université Paris-Saclay, CEA, INRAE, Médicaments et Technologies pour la Santé (MTS), SPI, Gif-sur-Yvette, France. karla.pereztoralla@cea.fr.

Methods in Molecular Biology (Clifton, N.J.)
|May 16, 2024
PubMed
Summary

This study introduces a novel microfluidic method for isolating circulating cell-free DNA (cfDNA) from blood samples. The METRO protocol efficiently captures fragmented tumor DNA for noninvasive cancer detection.

Keywords:
CancerCirculating cell-free DNACirculating tumor DNAMicrofluidic fluidized bedSolid-phase extraction

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Circulating cell-free DNA (cfDNA) analysis is crucial for noninvasive cancer monitoring.
  • Low cfDNA abundance and high fragmentation pose challenges for isolation.
  • Existing methods struggle with raw biological samples like undiluted serum.

Purpose of the Study:

  • To develop an efficient protocol for isolating circulating cfDNA from minimal sample volumes.
  • To overcome limitations of current cfDNA extraction methods.
  • To enable sensitive downstream applications like digital PCR.

Main Methods:

  • A dynamic Magnetic ExTRactiOn (METRO) protocol utilizing microfluidic fluidized bed technology.
  • Maximizing DNA binding surface area within a microfluidic chip.
  • Processing raw biological materials, including undiluted serum, with minimal reagents.

Main Results:

  • Successful isolation of cfDNA from small sample volumes (μL).
  • Efficient capture of short, fragmented DNA molecules.
  • Protocol is automatable and compatible with droplet-based digital PCR (ddPCR).

Conclusions:

  • The METRO protocol offers a promising advancement in cfDNA isolation.
  • This method enhances noninvasive cancer detection and personalized medicine.
  • The protocol's efficiency and compatibility support sensitive molecular diagnostics.