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Updated: Apr 26, 2026

High-Throughput, Multi-Image Cryohistology of Mineralized Tissues

High-Throughput, Multi-Image Cryohistology of Mineralized Tissues

Published on: September 14, 2016

High-throughput histopathology for complex in vitro models.

Marius F Harter¹, Elisa D'Arcangelo², Julien Aubert²

¹Institute of Human Biology (IHB), Roche Pharma Research and Early Development, Roche Innovation Center Basel, Basel, Switzerland; Gustave Roussy Cancer Campus, University Paris-Saclay, Paris, France.

Cell Reports Methods

|April 24, 2026

Summary

This summary is machine-generated.

This study introduces 3D-printed histomolds for high-throughput histology of complex in vitro models (CIVMs). This novel workflow streamlines sample processing, reducing labor and reagent costs for broader accessibility in histopathology.

Keywords:

CP: imaging CP: stem cell FFPE embedding of complex in vitro models high-throughput embedding histopathology immunohistochemistry microarrayed tissues multiplex immunofluorescence organoid FFPE sections organoids

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Area of Science:

Histopathology
Biotechnology
In vitro modeling

Background:

Histology is crucial for characterizing complex in vitro models (CIVMs).
Current histological workflows are low-throughput and time-consuming.
There is a need for efficient methods to handle multiple CIVMs simultaneously.

Purpose of the Study:

To develop a high-throughput histology workflow for CIVMs.
To introduce 3D-printed histomolds for co-planar sample embedding.
To reduce labor and reagent costs in CIVM analysis.

Main Methods:

Development of model-specific 3D-printed histomolds.
Co-planar embedding of up to 80 CIVMs per section.
High-plex staining and image analysis of embedded samples.
Description of the complete "histo-workflow" from mold generation to analysis.

Main Results:

Successful implementation of a high-throughput "histo-workflow" using histomolds.
Demonstrated spatially controlled histological sectioning of various CIVMs.
Significant reduction in labor and reagent resources achieved.
Up to 80 samples processed in a single section.

Conclusions:

3D-printed histomolds enable high-throughput processing and analysis of CIVMs.
The developed workflow democratizes high-throughput handling of CIVMs in histopathology.
This method offers a scalable and cost-effective solution for CIVM characterization.