Saccharomonospora saliphila sp. nov., a halophilic actinomycete from an Indian soil

A Gram-positive, moderately halophilic actinomycete strain, designated YIM 90502(T), was isolated from a sample of muddy soil collected from Gulbarga, Karnataka Province, India, and subjected to a polyphasic taxonomical analysis. The isolate grew optimally at 28 degrees C and in the presence of 10 % (w/v) NaCl. The isolate was characterized chemotaxonomically as having meso-diaminopimelic acid in the cell-wall peptidoglycan and galactose and arabinose as whole-cell sugars. The predominant menaquinone was MK-9(H(4)), while MK-8(H(4)) was found in smaller amounts. The phospholipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were iso-C(16 : 0) (49.2 %) and C(17 : 1)omega6c (9.1 %). The DNA G+C content of the isolate was 71.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the isolate fell within the evolutionary radiation encompassed by the genus Saccharomonospora. 16S rRNA gene sequence similarity between strain YIM 90502(T) and the type strains of Saccharomonospora species ranged from 92.42 % (with Saccharomonospora xinjiangensis CCTCC AA 97021(T)) to 97.45 % (with Saccharomonospora azurea KCTC 9693(T)). Levels of DNA-DNA relatedness between strain YIM 90502(T) and S. azurea KCTC 9693(T), Saccharomonospora halophila DSM 44411(T) and Saccharomonospora paurometabolica DSM 44619(T) were 46.0, 41.0 and 42.5 %, respectively. On the basis of phenotypic, phylogenetic and genotypic data, strain YIM 90502(T) was classified in the genus Saccharomonospora as a member of a novel species, for which the name Saccharomonospora saliphila sp. nov. is proposed, with YIM 90502(T) (=KCTC 19234(T) =DSM 45087(T)) as the type strain.