Jove
Visualize
Contact Us

Related Experiment Videos

Localization of the heparin-binding site on complement factor H.

M K Pangburn1, M A Atkinson, S Meri

  • 1Department of Biochemistry, University of Texas Health Science Center, Tyler 75710.

The Journal of Biological Chemistry
|September 5, 1991
PubMed
Summary

Factor H binds polyanions like heparin via a site in domain 13, distinct from its complement regulatory site. This binding influences Factor H

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipopolysaccharides and outer membrane proteins as main structures involved in complement evasion strategies of non-typhoidal Salmonella strains.

Molecular immunology·2022
Same author

Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1.

Nature communications·2021
Same author

Dysfunction of complement receptors CR3 (CD11b/18) and CR4 (CD11c/18) in pre-eclampsia: a genetic and functional study.

BJOG : an international journal of obstetrics and gynaecology·2021
Same author

Histological validation of a type 1 diabetes clinical diagnostic model for classification of diabetes.

Diabetic medicine : a journal of the British Diabetic Association·2020
Same author

Tumour-cell-derived complement components C1r and C1s promote growth of cutaneous squamous cell carcinoma.

The British journal of dermatology·2019
Same author

Untargeted metabolomic analysis in non-fasted diabetic dogs by UHPLC-HRMS.

Metabolomics : Official journal of the Metabolomic Society·2019
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Immunology
  • Biochemistry
  • Complement System

Background:

  • Factor H regulates complement activation, preventing self-attack by binding host cell markers.
  • Understanding Factor H's host recognition site is crucial for its function.

Purpose of the Study:

  • To characterize the binding properties of Factor H for polyanions.
  • To identify the specific site on Factor H responsible for host recognition and polyanion binding.

Main Methods:

  • Heparin-agarose binding assays were used to assess Factor H affinity for various polyanions.
  • Photoaffinity labeling with a heparin probe identified the approximate binding region.
  • Affinity chromatography and CNBr cleavage were employed to pinpoint the precise binding domain.

Main Results:

  • Factor H binds strongly to heparin, heparan sulfate, and dextran sulfate, but not polyaspartic acid or colominic acid.
  • The polyanion-binding site was localized to the region between domains 12 and 15, specifically suggesting domain 13.
  • This polyanion-binding site is spatially separated from the C3b-binding site (domains 4-6) by over 200 Å.

Conclusions:

  • Domain 13 of Factor H contains the primary site for polyanion binding.
  • This polyanion-binding site is distinct from the C3b-binding site, suggesting a complex regulatory mechanism.
  • The findings elucidate the molecular basis of Factor H's interaction with host surfaces.

Related Experiment Videos