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Updated: Jul 6, 2026

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

Published on: August 13, 2021

The zebrafish pronephros: a model to study nephron segmentation.

R A Wingert¹, A J Davidson

¹Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. ajdavidson@partners.org

Kidney International

|March 7, 2008

Summary

This summary is machine-generated.

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Researchers discovered that retinoic acid signaling guides nephron segmentation in zebrafish, a process conserved in mammals. This finding offers insights into kidney development and diseases.

Area of Science:

Developmental Biology
Renal Biology
Genetics

Background:

Nephrons exhibit segmental organization crucial for solute transport.
Mechanisms controlling nephron segmentation are poorly understood.
Understanding segmentation can illuminate kidney diseases and birth defects.

Purpose of the Study:

To investigate the conserved developmental pathways controlling nephron segmentation.
To explore the role of retinoic acid signaling in pronephric nephron development.
To validate zebrafish as a model for studying mammalian nephrogenesis.

Main Methods:

Comparative embryology studies in zebrafish and Xenopus.
Analysis of retinoic acid signaling pathways.
Investigating gene expression of renal transcription factors.

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Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney

Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney

Published on: August 9, 2014

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Published on: April 7, 2016

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Last Updated: Jul 6, 2026

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

Published on: August 13, 2021

Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney

Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney

Published on: August 9, 2014

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Published on: April 7, 2016

Main Results:

Pronephric nephrons in zebrafish and Xenopus show similar segmentation to mammals.
Retinoic acid signaling establishes proximodistal segment identity in zebrafish.
Signaling pathways identified are conserved in mammalian nephrogenesis.

Conclusions:

Zebrafish serve as a valuable genetic model for conserved nephron segmentation pathways.
Retinoic acid signaling is a key regulator of nephron segment identity.
Findings advance understanding of kidney development and disease mechanisms.