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"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
Published on: February 19, 2019
[Proteomic analysis of hepatitis B virus-infected human liver tissues]
Xue Qin1, Li Xie, Xiao-yan Chen
1Central Laboratory, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
Objective:
To search for the difference of protein molecules expression of HBV infection.
Methods:
Specimens taken from human normal liver tissues (group A), HBV infected human liver tissues which were HBsAg positive, and HBsAg, anti-HBe, and anti-HBc positive in serum (group B) were analysed through the methods of 2-dimensional electrophoresis (2-DE) and matrix-assisted laser-desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS/MS).
Results:
Totally 1125 plus/minus 56 (n=3) spots were detected in the sample of group A, 1203 plus/minus 42 (n=3) in group B samples. The percent volume of the protein spots was compared to show the proteome alteration in HBV infected human liver tissues. Forty proteins were found to present variations of two or more than two fold in quantity and 22 differentially expressed protein sports were finally identified by MALDI-TOF-MS/MS, including human mitochondrial aldehyde dehydrogenase, haptoglobin Hp2, peroxiredoxin 2, etc.
Conclusion:
The protein profile of human normal liver tissue and HBV infected liver tissues showed obviously difference.
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