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[Research on method in avoiding the interference of poloxamer in determination of polysaccharides in thermosensitive
Ling-hai Jiang1, Yi Feng, Lan Shen
1Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Objective:
To study a method in avoiding the interference of poloxamer in determination of polysaccharides in thermosensitive gel and develop an assay correspondingly.
Methods:
After extracting the freeze dried gel with acetic ether, detected it in colorimetric method.
Results:
The interference of poloxamer was removed thoroughly, while the retention rate of the polysacchrarides showed to be 100.11% (n = 3). The average recovery was 102.6% (n = 6).
Conclusion:
The method shows to be simple, maneuverable, stable and well reproducible.
