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Updated: Jul 6, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
Published on: January 9, 2017
Experimental tests of different solutions to the diffusion equation for optical characterization of scattering media
I Delfino1, M Lepore, P L Indovina
1Dipartimento di Scienze Fisiche, Istituto Nazionale per la Fisica della Materia, Università di Napoli Federico II, 80100 Napoli, Italy. delfino@na.infn.it
Abstract:
A detailed investigation of the use of time-resolved trasmittance for the optical characterization of scattering media by use of different analytical solutions to the diffusion equation has been performed. A femtosecond Ti:sapphire laser working at 800 nm and a streak camera with a time resolution of a few picoseconds were employed. Different latex and Intralipid solutions as well as biological samples were investigated. Reduced scattering coefficients were evaluated, and good agreement with the Mie predictions was found. An estimation of the order of magnitude of the absorption coefficient was obtained for the low-absorbance samples examined. These studies confirm experimentally that time-resolved trasmittance can be employed usefully for evaluating s values of thick scattering samples when a proper theoretical description that takes into account realistic boundary conditions is used.

