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Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: Jul 6, 2026

High-speed Particle Image Velocimetry Near Surfaces
11:59

High-speed Particle Image Velocimetry Near Surfaces

Published on: June 24, 2013

High-speed, two-photon scanning microscope.

K H Kim1, C Buehler, P T So

  • 1Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. kimki@mit.edu

Applied Optics
|March 8, 2008
PubMed
Summary

We developed a high-speed two-photon microscope for real-time imaging. This advanced system offers submicrometer resolution and minimizes photodamage, enabling detailed cellular observation.

Area of Science:

  • Biomedical Engineering
  • Microscopy
  • Cell Biology

Background:

  • Conventional scanning microscopes have limitations in speed and resolution.
  • Real-time imaging of dynamic biological processes is crucial for understanding cellular function.
  • Minimizing photodamage during high-speed imaging is essential for specimen viability.

Purpose of the Study:

  • To develop a high-speed two-photon microscope with submicrometer resolution.
  • To achieve real-time imaging capabilities for biological specimens.
  • To reduce photodamage compared to traditional microscopy techniques.

Main Methods:

  • Utilized a high-speed polygonal mirror scanner for rapid image acquisition.
  • Employed a two-photon excitation strategy for deeper tissue penetration and reduced scattering.

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In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
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In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

Published on: January 19, 2011

Related Experiment Videos

Last Updated: Jul 6, 2026

High-speed Particle Image Velocimetry Near Surfaces
11:59

High-speed Particle Image Velocimetry Near Surfaces

Published on: June 24, 2013

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
14:26

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

Published on: January 19, 2011

  • Recorded high-resolution fluorescence images using an intensified CCD camera.

    • Main Results:

    • Achieved imaging speeds approximately 100 times faster than conventional microscopes (40 micros/line).
    • Resolved cellular architecture in three dimensions with submicrometer resolution in real time.
    • Successfully monitored the movement of protozoa, demonstrating the system's dynamic imaging capabilities.

    Conclusions:

    • The developed high-speed two-photon microscope enables unprecedented real-time, high-resolution imaging of biological samples.
    • Two-photon excitation significantly minimizes photodamage, preserving specimen integrity during video-rate observation.
    • This technology advances the study of cellular dynamics and three-dimensional structures.