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Published on: June 23, 2022
Stimuli-responsive hydrogel-silver nanoparticles composite for development of localized surface plasmon
Tatsuro Endo1, Ryuzoh Ikeda, Yasuko Yanagida
1Department of Mechano-Micro Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan. endo.t.ab@m.titech.ac.jp
This study developed a novel optical biosensor for glucose detection. The localized surface plasmon resonance (LSPR) biosensor utilizes a stimuli-responsive hydrogel with silver nanoparticles for highly sensitive and cost-effective medical testing.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Localized surface plasmon resonance (LSPR) offers sensitive optical detection.
- Stimuli-responsive hydrogels can modulate nanoparticle interactions.
- Silver nanoparticles are crucial for LSPR-based sensing applications.
Purpose of the Study:
- To develop a localized surface plasmon resonance (LSPR)-based optical enzyme biosensor.
- To utilize a stimuli-responsive hydrogel-silver nanoparticle composite for enhanced glucose detection.
- To demonstrate the potential for cost-effective and highly sensitive medical test kits.
Main Methods:
- Immobilization of glucose oxidase (GOx) into a stimuli-responsive hydrogel.
- Incorporation of silver nanoparticles within the hydrogel matrix.
- Monitoring LSPR absorbance changes in response to glucose concentration.
Main Results:
- Glucose presence increased interparticle distances of silver nanoparticles, decreasing LSPR absorbance.
- Hydrogen peroxide from enzymatic reaction degraded silver nanoparticles, causing significant LSPR changes.
- The biosensor specifically determined glucose concentrations with a detection limit of 10 pM.
Conclusions:
- The developed LSPR-based optical enzyme biosensor is effective for specific glucose determination.
- The biosensor exhibits high sensitivity, with a detection limit in the picomolar range.
- This technology holds potential for developing simplified, cost-effective, and sensitive medical test kits.
