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AcMNPV ac143 (odv-e18) is essential for mediating budded virus production and is the 30th baculovirus core gene

Christina B McCarthy1, David A Theilmann

  • 1Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada V0H 1Z0.

Virology
|March 11, 2008
PubMed

Insights

Autographa californica multiple nucleopolyhedrovirus ac143 gene is essential for budded virus production. Knockout viruses show single-cell infections and no budded virus, despite normal DNA replication.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac143, previously identified as a late gene (ODV-E18), encodes a predicted 9.6 kDa structural protein.
  • This protein localizes to the occlusion-derived viral envelope and intranuclear microvesicles.

Purpose of the Study:

  • To re-evaluate the role of the AcMNPV ac143 gene in the viral life cycle.
  • To determine if ac143 is essential for viral replication and budded virus (BV) production.

Main Methods:

  • Generated an ac143 knockout (KO) virus (AcBAC(ac142)(REP-ac143KO)) using the AcMNPV bacmid system.
  • Utilized fluorescence and light microscopy to observe viral infection progression.
  • Performed titration assays to quantify budded virus (BV) production.
  • Analyzed viral DNA replication and RNA transcription in KO virus-infected cells.
  • Constructed deletion mutants of the ac142-ac143 gene cluster.

Main Results:

  • AcMNPV ac143 is a core gene, not a late gene, and is essential for budded virus (BV) production.
  • Ac143 KO virus infection is restricted to single cells and fails to produce BV.
  • Occlusion body formation occurs in the late stages of infection, indicating progression beyond the BV production stage.
  • Viral DNA replication is not affected by the absence of ac143.
  • The ac142-ac143 core gene cluster, including the ac143 promoter within the ac142 open reading frame, is crucial for viral replication, BV production, and RNA transcription.

Conclusions:

  • AcMNPV ac143 is a critical core gene required for efficient budded virus production.
  • The ac142-ac143 gene cluster plays a vital role in multiple stages of the baculovirus life cycle, including replication and transcription.

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