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Viral morphogenesis and morphological changes in human neuronal cells following Tioman and Menangle virus infection

K C Yaiw1, A Hyatt, R Vandriel

  • 1Department of Pathology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Archives of Virology
|March 12, 2008
PubMed

Insights

Tioman virus (TioPV) and Menangle virus (MenPV) are related paramyxoviruses with zoonotic potential. Electron microscopy revealed distinct intracellular ultrastructural differences in infected human neuronal cells, aiding differentiation.

Area of Science:

  • Virology
  • Cell Biology
  • Neuroscience

Background:

  • Tioman virus (TioPV) and Menangle virus (MenPV) are antigenically and genetically related Rubulavirus paramyxoviruses.
  • Both TioPV and MenPV are potential zoonotic agents, necessitating further characterization.
  • Human neuronal cell lines offer a model for studying viral interactions with neural tissue.

Purpose of the Study:

  • To investigate and compare the infectivity, growth kinetics, morphology, and morphogenesis of TioPV and MenPV.
  • To examine the effects of TioPV and MenPV infection on a human neuronal cell (SK-N-SH) line.
  • To identify potential ultrastructural markers for differentiating TioPV and MenPV in infected neuronal cells.

Main Methods:

  • Infection of sub-confluent SK-N-SH cells with TioPV and MenPV at a similar multiplicity of infection.
  • Analysis using conventional and immunoelectron microscopy to assess viral morphology and cellular changes.
  • Assay of virus titres in cell culture supernatants to determine growth kinetics.

Main Results:

  • Syncytia formation observed in both TioPV and MenPV infected SK-N-SH cells, more pronounced in early TioPV infection.
  • Distinct differences in viral morphology and intracellular inclusions: TioPV showed spherical virions and large tubule-like inclusions, while MenPV exhibited pleomorphic virions with smaller, interspersed inclusions.
  • Differential cellular pathology and ultrastructural characteristics observed between TioPV and MenPV infections in neuronal cells.

Conclusions:

  • TioPV and MenPV exhibit distinct intracellular ultrastructural characteristics within infected human neuronal cells.
  • Electron microscopy can differentiate between TioPV and MenPV based on their unique morphologies and inclusion bodies.
  • These findings contribute to understanding paramyxovirus-host interactions in neuronal cells and aid in viral diagnostics.

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