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Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
Cellular uptake of fluorescent labelled biotin-streptavidin microspheres
M Bradley1, L Alexander, R M Sanchez-Martin
1Chemical Biology Section, School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh, UK.
Abstract:
Amino functionalized, cross-linked, polystyrene microspheres were covalently loaded with streptavidin to which was coupled fluorescently labeled biotin and biotinylated-tagged DNA. These biotin-streptavidin microsphere conjugates were then successfully delivered into cells. The application of the streptavidin-biotin technology to these microspheres allows the effective delivery of any biotinylated material into intact mammalian cells, without the need for delicate procedures such as micro-injection.

