Related Experiment Video
Updated: Jul 6, 2026

Fluorescence Imaging with One-nanometer Accuracy (FIONA)
Published on: September 26, 2014
Fluorescent two-photon nanolithography
D Kunik1, S J Ludueña, S Costantino
1Departamento de Física, FCEN, Universidad de Buenos Aires, Buenos Aires, Argentina. dkunik@df.uba.ar
Abstract:
Improving the excitation conditions in two-photon fluorescent lithography reduces the size of the fabricated structures to nanometre scales. We demonstrate that a precise control of the illumination profile and the scanning speed of the laser beam is enough to decrease the photo-polymerization volume of resins by orders of magnitude. This work also shows experimental evidence of surface effects that yield a different polymerization intensity threshold compared with bulk. Such phenomenon enables to perform a non-linear optical nanolithography in a simple way, allowing fast-prototyping procedures. We present a detailed study of the polymer growth process using fluorescence and atomic force microscopy.
Related Concept Videos
Super-resolution Fluorescence Microscopy
Confocal Fluorescence Microscopy
Two-Dimensional Microscopy in Microbiology
Total Internal Reflection Fluorescence Microscopy
Fluorescence and Phosphorescence: Instrumentation

