Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PAD2-mediated citrullination of MBP and early myelin destabilization in multiple sclerosis.

Journal of autoimmunity·2026
Same author

Biological and clinical characteristics of <i>ETV6</i>::<i>RUNX1</i>-like ALL.

HemaSphere·2026
Same author

UV-induced mutations accumulate during early clonal expansion in aneuploid subtypes of pediatric B-cell precursor acute lymphoblastic leukemia.

Haematologica·2026
Same author

First-year results of the International Leukemia/Lymphoma Target Board for pediatric relapsed and refractory hematological malignancies.

Haematologica·2026
Same author

KMT2A-rearranged B-lymphoblastic lymphomas are skewed towards a more mature developmental stage.

Leukemia·2026
Same author

From the bench of molecular understanding to the bedside of optimal therapy for <i>BCR</i>::<i>ABL1</i> and ABL-class acute lymphoblastic leukemia in children and adolescents.

EJC paediatric oncology·2026
Same journal

Genome-wide analysis across Indian camel populations reveals genetic distinctiveness of the Kharai camel breed.

BMC genomics·2026
Same journal

Different genomic footprint of small insertion-deletion and structural variants determines the genetic divergence of indica and japonica rice.

BMC genomics·2026
Same journal

From nurse bee to queen egg: RNA-seq analysis of Apis mellifera eggs shows dietary protein-dependent gene regulation.

BMC genomics·2026
Same journal

A genome-wide association study to identify the genetic loci underlying carbapenem resistance in Acinetobacter baumannii.

BMC genomics·2026
Same journal

Comparative transcriptome analysis to reveal key drought stress-responsive genes in sorghum (Sorghum bicolor (L.) Moench).

BMC genomics·2026
Same journal

Tissue identity is the dominant determinant of cross-species transferability of a porcine developmental programme.

BMC genomics·2026
See all related articles

Related Experiment Video

Updated: Jul 6, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Can subtle changes in gene expression be consistently detected with different microarray platforms?

Paola Pedotti1, Peter A C 't Hoen, Erno Vreugdenhil

  • 1Center for Human and Clinical Genetics, Leiden University Medical Center, Leiden, The Netherlands. paola.pedotti@gmail.com

BMC Genomics
|March 12, 2008
PubMed
Summary
This summary is machine-generated.

Comparing five microarray platforms revealed significant differences in detecting subtle gene expression changes. Using two platforms may offer complementary views and higher power for detecting differential gene expression, especially with limited biological variation.

More Related Videos

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
10:37

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

Related Experiment Videos

Last Updated: Jul 6, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
10:37

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Gene expression data comparability across microarray platforms remains a challenge.
  • Subtle differences in gene expression require sensitive detection methods.

Purpose of the Study:

  • To evaluate the performance and overlap of five microarray platforms in detecting differentially expressed genes.
  • To compare platform sensitivity in a biological context with minimal expression variations.

Main Methods:

  • Five microarray platforms (Applied Biosystems, Affymetrix, Agilent, Illumina, LGTC) were used to analyze hippocampal gene expression in wild-type and transgenic mice.
  • Differential gene expression analysis was performed using a fixed false discovery rate of 10%.
  • Quantitative RT-PCR was used to validate findings.

Main Results:

  • Significant variations in the number of differentially expressed genes were observed across platforms (ABI: 4, Affymetrix: 130, Agilent: 3,051, Illumina: 54, LGTC: 13).
  • Two genes were consistently detected by all platforms; four genes identified by ABI were found by at least three others.
  • Improved correlation between platforms was noted when ranking genes by significance level rather than a fixed cutoff.
  • Aberrations in GABA-ergic signaling were consistently identified across all platforms.

Conclusions:

  • Microarray platforms provide partially complementary insights into affected biological processes.
  • Employing two different platforms may be more advantageous than increasing replicates for subtle expression differences.
  • Commercial two-color platforms demonstrated higher power in detecting differential gene expression in groups with small expression variations.