Related Experiment Video
Updated: Jul 6, 2026

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
Published on: July 20, 2022
Urinary excretion study of coenzyme Q10 in rats by ultra-performance liquid chromatography-mass spectrometry
Lie Li1, Deepthi Pabbisetty, Shiqian Zhu
1Department of Pharmaceutics, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
Abstract:
A method based on ultra-performance liquid chromatography mass spectrometry (UPLC-MS) applying atmospheric pressure chemical ionization in the positive ion mode is developed for the determination of coenzyme Q10 (CoQ10) in rat urine. The assay involves the extraction of crude urine, fast liquid chromatography on a Waters Acquity UPLC BEH C18 column (1.7 microm, 1.0 x 50 mm), and selected ion monitoring detection using mass transition. The calibration range is found to be 0.05-25 microg/mL, with the lower limit of quantitation of 0.05 microg/mL. Intra- and inter-day precision (relative standard deviation) for CoQ10 in rat urine range from 0.7% to 15%, and accuracy expressed in recovery rates in urine is between 83% and 118%. The recovery of this method is found to be between 80% and 95% at three concentrations. The total cumulative recovery of CoQ10 is 1.16 +/- 1.05% (percentage of dose intake, n = 4) from rat urine collected over 30 h after oral administration of the drug. The UPLC-MS method described allows the quick determination of CoQ10 in rat urine with good precision and accuracy. It is suitable for further excretion studies of CoQ10 in animals.
Related Concept Videos
Urine Studies I: Urinalysis
One-Compartment Open Model: Urinary Excretion Data and Determination of k
Renal Drug Clearance: Comparison Between Renal Excretion Methods
Renal clearance is often associated with the renal glomerular filtration rate (GFR), which represents the rate at which plasma is filtered through the glomeruli in the kidney. When drug reabsorption is minimal and there is no active secretion, renal clearance is closely related to the...
Renal Drug Excretion: Tubular Secretion
Renal Drug Excretion: Effect of Urine pH, Flow Rate, and Drug pKa
The pKa of a drug,...
Physiology of the Genitourinary System III: Urine Concentration and Dilution
