Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>trans</i>-Translation inhibitors that kill <i>Mycobacterium tuberculosis</i> and pathogenic non-tuberculous mycobacteria also disrupt metal homeostasis.

Microbiology (Reading, England)·2026
Same author

Ribosomal Protein bL27 Protects Translating Ribosomes from tmRNA-SmpB.

bioRxiv : the preprint server for biology·2026
Same author

<i>trans</i>-Translation inhibitors that kill <i>M. tuberculosis</i> and pathogenic Non-tuberculous Mycobacteria have a dual mechanism of action.

bioRxiv : the preprint server for biology·2026
Same author

Targeting Recoding by <i>trans</i>-Translation to Develop Antibiotics.

Cold Spring Harbor perspectives in biology·2025
Same author

A <i>trans</i>-translation inhibitor that targets ribosomal protein bL12 kills <i>Mycobacterium tuberculosis</i>.

Journal of bacteriology·2025
Same author

A <i>trans</i> -translation inhibitor kills <i>Mycobacterium tuberculosis</i> by targeting ribosomal protein bL12.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jul 6, 2026

Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy
09:24

Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy

Published on: November 29, 2017

Screen for localized proteins in Caulobacter crescentus.

Jay H Russell1, Kenneth C Keiler

  • 1Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, United States of America.

Plos One
|March 13, 2008
PubMed
Summary

Scientists identified numerous localized bacterial proteins using a novel screening method. This technique reveals complex protein localization patterns and suggests short peptide sequences can direct protein location within cells.

More Related Videos

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
10:53

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo

Published on: November 7, 2013

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
11:31

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Published on: June 30, 2016

Related Experiment Videos

Last Updated: Jul 6, 2026

Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy
09:24

Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy

Published on: November 29, 2017

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
10:53

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo

Published on: November 7, 2013

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
11:31

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Published on: June 30, 2016

Area of Science:

  • Microbiology
  • Cell Biology
  • Bacterial Protein Localization

Background:

  • Precise protein localization is crucial for bacterial functions like motility and cell division.
  • The full extent of protein localization in bacteria remains largely unknown.
  • Understanding protein localization is key to deciphering bacterial cellular organization.

Purpose of the Study:

  • To develop and implement a high-throughput screen for identifying localized proteins in bacteria.
  • To discover novel proteins with specific localization patterns in Caulobacter crescentus.
  • To investigate the role of short peptides in bacterial protein targeting.

Main Methods:

  • Utilized a screening approach combining transposon mutagenesis and fluorescence-activated cell sorting (FACS).
  • Applied the screen to the bacterium Caulobacter crescentus to identify localized proteins.
  • Analyzed transposon insertion sites to understand protein localization signals.

Main Results:

  • Identified eleven previously uncharacterized localized proteins in C. crescentus, including six hypothetical proteins.
  • Discovered proteins with cell-cycle-dependent localization and helix-like structures.
  • Demonstrated that short peptide sequences can mediate protein localization, even with near 5' gene insertions.

Conclusions:

  • Complex three-dimensional and cell-cycle-dependent protein localization patterns are likely common in bacteria.
  • Short peptides contain sufficient information for bacterial protein localization.
  • The developed screening method is adaptable for use across diverse bacterial species.