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Updated: Jul 6, 2026

A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells
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A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells

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[Chemosensitivity test on brain tumors].

J L Gil-Salú1, J Bosco-López, M Domi Nguez-Villar

  • 1Servicios de Neurocirugía, Unidad Investigación, Hospital Universitario Puerta del Mar, Cádiz.

Neurocirugia (Asturias, Spain)
|March 13, 2008
PubMed
Summary
This summary is machine-generated.

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The MTT assay effectively measures in vitro chemosensitivity in brain tumors to new chemotherapy drugs, aiding in personalized cancer treatment selection.

Area of Science:

  • Oncology
  • Pharmacology
  • Biotechnology

Context:

  • Chemotherapy is crucial for cancer treatment, but predicting drug efficacy in specific cancers remains challenging.
  • In vitro chemosensitivity testing offers a promising approach to assess tumor response and optimize drug dosage for individual patients.
  • Brain tumors, including Glioblastoma multiforme, meningiomas, and medulloblastoma, require tailored treatment strategies due to their complex nature.

Purpose:

  • To evaluate the sensitivity of various brain tumor types to a panel of antineoplastic drugs.
  • To validate the MTT assay as a reliable method for determining in vitro chemosensitivity in brain tumors.
  • To assess the dose-response relationship of selected chemotherapy agents against primary brain tumor cultures.

Summary:

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Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging
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Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging

Published on: July 13, 2010

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Last Updated: Jul 6, 2026

A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells
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A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells

Published on: March 28, 2021

Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging
11:09

Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging

Published on: July 13, 2010

  • Five chemotherapy drugs (carmustine, camptothecin, paclitaxel, hydroxyurea, and tamoxifen) were tested on short-term cultures from 23 brain tumor patients.
  • The MTT assay was employed to measure chemosensitivity, with optical density read at 450 nm to generate dose-result curves.
  • Valid dose-response curves (IC50) were obtained for each drug, representing tumor sensitivity.

    • Impact:

    • The study validates the MTT assay as a robust tool for assessing in vitro chemosensitivity in brain tumors.
    • Findings support the use of MTT assays for predicting patient response to novel chemotherapy agents.
    • This method can potentially guide the selection of optimal chemotherapy regimens for brain tumor patients, improving treatment outcomes.