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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...

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Polysome Purification from Soybean Symbiotic Nodules
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Polysome Purification from Soybean Symbiotic Nodules

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Transcriptomics.

Virendra S Gomase1, Somnath Tagore

  • 1Department of Bioinformatics, Dr. D.Y. Patil Institute for Biotechnology and Bioinformatics, Padmashree Dr. D.Y. Patil University, Plot No-50, Sector-15, CBD Belapur, Navi Mumbai, 400614, India. virusgene1@yahoo.co.in

Current Drug Metabolism
|March 14, 2008
PubMed
Summary
This summary is machine-generated.

Transcriptomics measures all messenger RNA (mRNA) transcripts in cells using DNA microarray technology. This field integrates genomics and proteomics for comprehensive biological insights.

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Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
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Last Updated: Jul 6, 2026

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Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

Area of Science:

  • Molecular Biology
  • Genomics
  • Proteomics

Background:

  • Transcriptomics analyzes genome-wide messenger RNA (mRNA) expression levels.
  • It encompasses the complete set of transcripts within cells or organisms.
  • This field builds upon existing technologies in genomics and proteomics.

Purpose of the Study:

  • To define transcriptomics and its scope in biological research.
  • To highlight its foundational technologies and interdisciplinary nature.

Main Methods:

  • Utilizes DNA microarray technology for gene expression profiling.
  • Involves the measurement of mRNA levels across a genome.

Main Results:

  • Provides a comprehensive snapshot of cellular transcriptional activity.
  • Enables the study of gene expression patterns in various biological contexts.

Conclusions:

  • Transcriptomics is a pivotal field for understanding cellular function.
  • Its integration with other 'omics' fields offers powerful research capabilities.