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Related Experiment Videos

Effective path length in attenuated total reflection spectroscopy.

Lacey A Averett1, Peter R Griffiths, Koichi Nishikida

  • 1Department of Chemistry, University of Idaho, Moscow, Idaho 83844-2343, USA.

Analytical Chemistry
|March 18, 2008
PubMed
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Attenuated total reflection (ATR) spectroscopy is widely used, but effective path length (d(e)) is more accurate than depth of penetration (d(p)). Modifying refractive index for anomalous dispersion is crucial for precise ATR measurements of viscous liquids.

Area of Science:

  • Spectroscopy
  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Attenuated total reflection (ATR) spectroscopy is a prevalent method for analyzing condensed phase samples.
  • Current practice often relies on the depth of penetration (d(p)) to estimate evanescent wave path length.
  • The effective path length (d(e)) offers a more accurate representation of the evanescent wave's interaction with the sample.

Purpose of the Study:

  • To investigate the accuracy of effective path length (d(e)) calculations in ATR spectroscopy.
  • To determine the necessity of modifying refractive index for accurate measurements of strongly absorbing viscous liquids.
  • To establish a more precise method for measuring band absorptivities in ATR spectroscopy.

Main Methods:

  • Utilized attenuated total reflection (ATR) spectroscopy for infrared spectral measurements.

Related Experiment Videos

  • Employed the concept of effective path length (d(e)) as a metric for evanescent wave interaction.
  • Investigated the impact of anomalous dispersion on refractive index values in viscous liquid samples.

    • Main Results:

    • Demonstrated that effective path length (d(e)) is a superior metric compared to depth of penetration (d(p)).
    • Showed that modifying the refractive index to account for anomalous dispersion is essential for accurate d(e) calculations.
    • Achieved more precise measurements of band absorptivities for strongly absorbing viscous liquids.

    Conclusions:

    • Effective path length (d(e)) provides a more accurate measure of evanescent wave interaction in ATR spectroscopy.
    • Accurate ATR measurements of viscous liquids require refractive index modification due to anomalous dispersion.
    • The study refines ATR methodology for precise quantitative analysis of challenging samples.