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A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
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Directed evolution of transketolase substrate specificity towards an aliphatic aldehyde

Edward G Hibbert1, Tarik Senussi, Mark E B Smith

  • 1Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.

Journal of Biotechnology
|March 18, 2008
PubMed
Summary

Directed evolution created transketolase (TK) mutants with enhanced specificity for propionaldehyde. Mutations at conserved residues improved activity on non-natural substrates, guiding enzyme engineering for specific applications.

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