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Updated: Jul 6, 2026

Mucociliary Epithelial Organoids from Xenopus Embryonic Cells: Generation, Culture and High-Resolution Live Imaging
Published on: July 28, 2020
Multicellular dynamics during epithelial elongation
Jennifer A Zallen1, J Todd Blankenship
1Developmental Biology Program, Sloan-Kettering Institute, New York, NY 10065, USA. zallenj@mskcc.org
Abstract:
The reorganization of multicellular populations to produce an elongated tissue structure is a conserved mechanism for shaping the body axis and several organ systems. In the Drosophila germband epithelium, this process is accompanied by the formation of a planar polarized network of junctional and cytoskeletal proteins in response to striped patterns of gene expression. Actomyosin cables and adherens junctions are dynamically remodeled during intercalation, providing the basis for polarized cell behavior. Quantitative analysis of cell behavior in living embryos reveals unexpected cell population dynamics that include the formation of multicellular rosette structures as well as local neighbor exchange.
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