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Sequence and localization of the gene encoding yeast phosphoglycerate mutase
J Heinisch1, R C von Borstel, R Rodicio
1Department of Genetics, University of Alberta, Edmonton, Canada.
Current Genetics
|July 1, 1991
Summary
Researchers sequenced the yeast phosphoglycerate mutase gene (GPM1), revealing regulatory elements and a unique repetitive sequence. The GPM1 gene was mapped to chromosome XI, providing insights into yeast genetics.
Area of Science:
- Molecular Biology
- Yeast Genetics
- Gene Regulation
Background:
- The phosphoglycerate mutase gene (GPM1) is crucial for glycolysis in yeast.
- Understanding gene structure and regulation is key to yeast metabolic studies.
Purpose of the Study:
- To determine the complete nucleotide sequence of the yeast GPM1 gene.
- To identify essential 5' and 3' non-coding regions and regulatory elements.
- To map the genomic location of the GPM1 gene.
Main Methods:
- S1-mapping to determine transcriptional start points.
- cDNA clone sequencing.
- Analysis of upstream and downstream DNA sequences.
- Gene mapping using genetic markers.
Main Results:
- Complete nucleotide sequence of the yeast GPM1 gene and its non-coding regions determined.
- Transcriptional start points identified.
- Presence of known yeast promoter regulatory sequences upstream of the GPM1 gene.
- A composite repetitive element, likely formed by recombination, found 3' to the coding region.
- GPM1 gene mapped 13 cM distal to fas1 on chromosome XI.
Conclusions:
- The GPM1 gene sequence provides a foundation for further studies on its regulation and function.
- The identified repetitive element offers insights into recombination mechanisms in yeast.
- Genomic mapping of GPM1 aids in understanding yeast chromosome organization and gene linkage.