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Multiple regulatory elements control the expression of the yeast ACR1 gene
B Redruello1, E Valdes, M Luz Lopez
1Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Oviedo, Spain.
FEBS Letters
|March 27, 1999
Summary
The ACR1 gene in Saccharomyces cerevisiae is crucial for ethanol metabolism, with its expression regulated by specific promoter elements responding to carbon sources and stress. These findings clarify yeast
Area of Science:
- Molecular biology
- Yeast genetics
- Transcriptional regulation
Background:
- The ACR1 gene in Saccharomyces cerevisiae encodes a succinate-fumarate transporter essential for ethanol utilization.
- ACR1 gene expression is upregulated by ethanol and downregulated by glucose, indicating carbon source-dependent regulation.
Purpose of the Study:
- To investigate the role of specific carbon source response elements (CSREs) and stress response promoter elements (SRPEs) in the transcriptional regulation of the ACR1 gene.
- To confirm the functional significance of these elements in controlling ACR1 gene expression.
Main Methods:
- Deletion analysis of ACR1 promoter regions containing CSREs and SRPEs.
- Functional assessment of these elements in a heterologous promoter system.
- Competition assays to evaluate protein binding to promoter elements.
Main Results:
- Three CSREs in the ACR1 promoter were confirmed to play a role in transcriptional regulation.
- These CSREs demonstrated varying abilities to compete for protein binding with elements from the ICL1 promoter.
- Two putative SRPEs in the ACR1 promoter were found to contribute to gene expression.
Conclusions:
- The ACR1 gene's expression is precisely controlled by multiple promoter elements, including CSREs and SRPEs.
- These regulatory elements enable yeast to adapt its metabolic machinery in response to different carbon sources and environmental stresses.