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Updated: Jul 6, 2026
Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis
Published on: November 18, 2019
[Identification of metastasis-associated proteins of ovarian cancer by proteomics]
Le Zhou1, Xiao-Feng Yang, Yan Wang
1The Key Laboratory of Biomedical Information Engineering of National Administration of Education, Institute for Cancer Research, Xi'an Jiaotong University, Xi'an, China.
Objective:
To investigate the differenal protein expression profiles of ovarian tumor cell lines with distinct metastatic abilities.
Methods:
The ovarian cancer cell line HO8910 and HO8910pm, derived from same parental cells but exhibited different metastatic ability, were investigated by two-dimensional gel electrophoresis (2-DE)-MALDI-TOF-MS proteomic approach.
Results:
Thirty-nine proteins were detected by 2-DE to have expression disparity levels over 2 folds between two cell lines. Eighteen of them were identified by MALDI-TOF-MS. The proteins are involved in apoptosis, extra cellular matrix (ECM), cytoskeleton, growth factor, glycolysis, protein metabolism and immune system.
Conclusion:
The data are valuable for the identification of differentially expressed proteins involved in the biological behavior of human ovarian cancer.
