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Related Concept Videos

Kidney Transplant I: Introduction01:28

Kidney Transplant I: Introduction

A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
Kidney Transplant II: Surgical Procedure01:26

Kidney Transplant II: Surgical Procedure

Preoperative ManagementThe primary goals of preoperative management in kidney transplantation are to optimize the patient’s metabolic state and prepare them for surgery through diet adjustments, necessary dialysis, and tailored medical treatment. This phase also involves comprehensive infection screening and patient education about the surgical procedure and postoperative care to improve outcomes and adherence.Medical ManagementA comprehensive evaluation is required for both the living donor...
Kidney Transplant III: Nursing Management01:16

Kidney Transplant III: Nursing Management

Postoperative Nursing Management for Kidney Transplant PatientsPostoperative nursing management care includes monitoring the surgical site, encouraging early movement, and promoting lung health through breathing exercises. Nurses also administer prescribed medications like H2-blockers, such as famotidine, or proton pump inhibitors, like omeprazole, to help prevent gastrointestinal ulcers and bleeding. Fungal infections in the mouth and bladder can result from immunosuppressive and antibiotic...
Tissue Transplantation01:24

Tissue Transplantation

Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...

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Related Experiment Video

Updated: Jul 6, 2026

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
08:12

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation

Published on: July 15, 2015

Gene expression patterns in deceased donor kidneys developing delayed graft function after kidney transplantation.

Valeria R Mas1, Kellie J Archer, Kenneth Yanek

  • 1Division of Transplant, Department of Surgery, Virginia Commonwealth University, Richmond, VA, USA.

Transplantation
|March 19, 2008
PubMed
Summary

Delayed graft function (DGF) after kidney transplantation is linked to specific gene expression changes in deceased donor kidneys. Identifying these genes, particularly those in inflammatory response pathways, can improve transplant outcomes.

Related Experiment Videos

Last Updated: Jul 6, 2026

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
08:12

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation

Published on: July 15, 2015

Area of Science:

  • Nephrology
  • Transplantation Immunology
  • Genomics

Background:

  • Delayed graft function (DGF) affects 2-50% of kidney transplants and negatively impacts long-term outcomes.
  • Understanding DGF mechanisms is crucial for improving kidney transplant success.
  • This study investigated gene expression in deceased donor kidney (DDK) biopsies to identify DGF-related factors.

Purpose of the Study:

  • To identify gene expression profiles associated with DGF in DDK.
  • To determine if specific genes or pathways predict DGF development.
  • To explore the relationship between cold ischemia time and DGF.

Main Methods:

  • Gene expression profiling using microarrays on 33 DDK biopsies (12 DGF, 21 non-DGF).
  • DGF defined as requiring dialysis within the first week post-transplant.
  • Logistic regression analysis to identify genes significantly associated with DGF, adjusting for cold ischemia time.

Main Results:

  • 36 genes were univariably differentially expressed between DGF and non-DGF groups.
  • 65 genes were differentially expressed after adjusting for cold ischemia time.
  • Overexpressed genes in DGF kidneys were linked to cell cycle, signal transduction, immune response (e.g., CD83, BCL3, MX1), and metabolism.

Conclusions:

  • Cold ischemia time is an independent predictor of DGF.
  • A panel of 36 candidate genes for DGF in DDK was identified.
  • Genes involved in the inflammatory response appear particularly important in DGF development.