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Related Experiment Videos

The thymus leukemia antigen binds human and mouse CD8.

M Teitell1, M F Mescher, C A Olson

  • 1Department of Microbiology and Immunology, University of California Los Angeles School of Medicine 90024.

The Journal of Experimental Medicine
|November 1, 1991
PubMed
Summary
This summary is machine-generated.

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Thymus leukemia antigen (TLA), a nonclassical MHC class I molecule, binds to CD8. This interaction supports CD8-dependent immune responses, suggesting TLA presents antigens to T cells, particularly in the gut.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Thymus leukemia antigen (TLA) is a nonclassical MHC class I molecule.
  • Its function remains largely unknown, with speculation about its role in antigen presentation to gamma delta T cell receptors (TCRs).

Purpose of the Study:

  • To investigate the interaction between TLA and CD8.
  • To determine if TLA binding to CD8 has functional consequences for T cell responses.

Main Methods:

  • Cell-cell adhesion assays were performed.
  • Adhesion of T lymphocyte clones to purified plate-bound TLA was assessed.
  • A chimeric class I molecule was engineered to study CD8-dependent responses.

Main Results:

Related Experiment Videos

  • TLA was shown to bind to both human and mouse CD8.
  • A chimeric molecule demonstrated CD8-dependent immune response support.
  • This is the first demonstration of a class Ib molecule binding to CD8 with functional outcomes.

    • Conclusions:

    • The conserved ability of TLA to interact with CD8, despite sequence divergence, highlights the significance of this interaction.
    • TLA's expression in the mouse small intestine suggests a role in antigen presentation to CD8+ intraepithelial lymphocytes, potentially including gamma delta TCR-bearing cells.